Gas-Chromatography/Gas-Chromatography with Mass Spectrometry

A gas-chromatograph coupled with a mass spectrometer (GC-MS) is a combination of two techniques. The gas chromatograph separates the components of a mixture in time, and the mass spectrometer provides information that aids in the structural identification of each component. Compounds that are nonpolar, volatile, and have a small molecular weight are suitable for analysis by GC. For non-volatile molecules, derivatization is needed and the sample should be metal free.

Instrument In charge: Dr. Basab Bijayi Dhar

Instrument Details:

GC: Agilent 7890B GC system equipped with an HP-5 capillary column (30 m × 0.32 mm × 0.25 μm)

GC-MS: Agilent 7890B GC system connected with 5977B GC/MSD equipped with an HP-5 MS capillary column (30 m × 0.32 mm × 0.25 μm). Agilent Mass Hunter and classic Agilent MSD ChemStation software is used to analyse the data and exact mass corrected to the first decimal.

Sample preparation:

For GC/GC-MS sample prepration, user have to use a simple filtration technique to remove solid particles and inorganic salts if you are using a reaction mixture. The solution should be very diluted (0.1 mg/mL). It is recommended that your analyte must have a molecular weight of less than 500 g/mol for GC-MS analysis.

Location: A203 (Catalysis lab)