Chemistry Department wins FIST grant from DST

Research
28 Mar 2018
  • MALDI ToF

The Department of Chemistry has been awarded a FIST grant (duration 5 years) from DST to augment the research facilities in the Department by acquisition of a MALDI ToF Mass Spectrometer.

The Total value of the FIST grant is Rs.2.64 crores.

The Department of Chemistry has been awarded a FIST grant (duration 5 years) from DST to augment the research facilities in the Department by acquisition of a MALDI ToF (Matrix Assisted Laser Desorption/Ionization Time of Flight) Mass Spectrometer.

MALDI Imaging: One main purpose for proposing to acquire this instrument is “imaging”. The MALDI technology has recently being used successfully for imaging of molecules and proteins in tissue and whole animal sections. The power of this technology is immense because, unlike the commonly used imaging methods, no fluorescence, radioactive or chemiluminescent probe is required here. As long as a molecule can be detected by MALDI, its tissue or whole body localization can be visualized by this technology. In addition, Bruker has developed software that allows construction of 3D images from data collected on multiple tissue samples across a horizontal cross-section of the sample. The main strength of this technology, however is that once the data is collected, unlike normal imaging methods, it is not restricted to a single molecule. The data is rich and can be processed for any other molecule in the sample if it is detectable by MALDI. Taken together, this instrument will vastly expand our research and imaging capabilities.

The total amount of the DST FIST grant is Rs.2.64 crores.

  • MALDI mass spectrometry imaging is able to simultaneously determine the spatial distribution of hundreds of molecules directly from tissue sections, without labeling and without prior knowledge.
  • No need for antibodies, radioactivity or fluorescence/chemiluminescence tags
  • Data collected once can be used for any detectable molecules

Applications of MALDI ToF Mass Spectrometer:

  • Drug and metabolite distribution in tissue

Visualization of drugs and metabolites in tissue. Unlike autoradiography, it can differentiate drugs and metabolites. This, combined with the microscopic information, opens an entirely a new level of histopharmacology.

Clincial cancer research

Cancer tissue is often very heterogenous, with many different cell types being present in various ratios. MALDI Imaging allows access to the complete spatial and histological information.

Metabolomics

Many endogenous metabolites and lipids can be imaged using MALDI. For many of these compounds, mass spectrometric imaging is the only way to investigate their spatial distribution.

Chemical crosstalk of microbial colonies

An interesting application of MALDI Imaging is the analysis of chemical crosstalk of microbial colonies.

 

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