Genomics and Proteomics facility
[I] Sample Preparation
Sonicator / Tissue Homogenizer:
- Efficient for cell lysis, protein extraction, and tissue homogenization.
- Ensures uniform sample preparation for accurate molecular profiling.
- Critical for downstream proteomic and genomic analyses.


2. Microcentrifuge: For small volume sample processing.
- Used for high-speed spinning of small samples, typically in microcentrifuge tubes.
- Essential for pelleting cells, nucleic acids, or proteins.
- Helps separate components for PCR, RNA extraction, and protein assays.


3. High-Speed Centrifuge: For larger volume samples.
- Enables high-speed separations in larger tubes.
- Ideal for pelleting organelles and density gradient centrifugation.
- Crucial for virus particles and nucleic acid purification.


4. Spectrophotometer (NanoDrop)
- Measures nucleic acid/protein concentrations in 1-2 µL samples.
- Assesses DNA/RNA purity (260/280 nm ratio).
- Convenient for high-throughput analysis.


5. Fluorometer (Qubit)
- Quantifies nucleic acids and proteins using fluorescent dyes.
- High sensitivity for low-concentration samples.
- Greater accuracy than spectrophotometers.

6. TapeStation
- Analyzes DNA/RNA quality via chip-based electrophoresis.
- Provides size distribution and RNA/DNA integrity.

7. Plate Reader
- High-throughput analysis of absorbance, fluorescence, luminescence.
- Ideal for enzyme, viability, and expression assays.

[II] PCR & Amplification
1. Thermocycler (PCR Machine)
- Performs DNA amplification through PCR.
- Used in gene cloning, mutagenesis, and genetic analysis.


2. Real-Time PCR (qPCR) System
- Quantifies DNA in real-time using fluorescence.
- Ideal for gene expression and viral load studies.


3. Electrophoresis System
- Separates DNA/RNA (horizontal) or proteins (vertical).
- Available in small, standard, and long formats.

4. Gel Documentation System
- Captures gel images with UV/LED light.
- Used for DNA, RNA, and protein visualization.



[III] Next-Generation Sequencing (NGS) & Genomics
1. MGI NGS Sequencer
- Performs high-throughput DNA/RNA sequencing.
- Used for genome, transcriptome, and targeted sequencing.

2. Oxford Nanopore
- Real-time, long-read sequencing.
- Ideal for genome assembly and structural variants.

[IV] Bioinformatics & Data Analysis
- High-Performance Computing (HPC) Server
- Workstations with Genome Analysis Software

[V] Protein Separation & Characterization
FPLC
- Purifies proteins based on size, charge, or affinity.
- Used for enzymes, antibodies, recombinant proteins.

[VI] High-Throughput Proteomics
Mass Spectrometry
- Analyzes molecules based on mass-to-charge ratio.
- Used for proteomics, lipidomics, metabolomics.
