Genomics and Proteomics facility

[I] Sample Preparation

Sonicator / Tissue Homogenizer:

  • Efficient for cell lysis, protein extraction, and tissue homogenization.
  • Ensures uniform sample preparation for accurate molecular profiling.
  • Critical for downstream proteomic and genomic analyses.

2. Microcentrifuge: For small volume sample processing.

  • Used for high-speed spinning of small samples, typically in microcentrifuge tubes.
  • Essential for pelleting cells, nucleic acids, or proteins.
  • Helps separate components for PCR, RNA extraction, and protein assays.

3. High-Speed Centrifuge: For larger volume samples.

  • Enables high-speed separations in larger tubes.
  • Ideal for pelleting organelles and density gradient centrifugation.
  • Crucial for virus particles and nucleic acid purification.

4. Spectrophotometer (NanoDrop)

  • Measures nucleic acid/protein concentrations in 1-2 µL samples.
  • Assesses DNA/RNA purity (260/280 nm ratio).
  • Convenient for high-throughput analysis.

5. Fluorometer (Qubit)

  • Quantifies nucleic acids and proteins using fluorescent dyes.
  • High sensitivity for low-concentration samples.
  • Greater accuracy than spectrophotometers.

6. TapeStation

  • Analyzes DNA/RNA quality via chip-based electrophoresis.
  • Provides size distribution and RNA/DNA integrity.

7. Plate Reader

  • High-throughput analysis of absorbance, fluorescence, luminescence.
  • Ideal for enzyme, viability, and expression assays.

[II] PCR & Amplification

1. Thermocycler (PCR Machine)

  • Performs DNA amplification through PCR.
  • Used in gene cloning, mutagenesis, and genetic analysis.

2. Real-Time PCR (qPCR) System

  • Quantifies DNA in real-time using fluorescence.
  • Ideal for gene expression and viral load studies.

3. Electrophoresis System

  • Separates DNA/RNA (horizontal) or proteins (vertical).
  • Available in small, standard, and long formats.

4. Gel Documentation System

  • Captures gel images with UV/LED light.
  • Used for DNA, RNA, and protein visualization.

[III] Next-Generation Sequencing (NGS) & Genomics

1. MGI NGS Sequencer

  • Performs high-throughput DNA/RNA sequencing.
  • Used for genome, transcriptome, and targeted sequencing.

2. Oxford Nanopore

  • Real-time, long-read sequencing.
  • Ideal for genome assembly and structural variants.

[IV] Bioinformatics & Data Analysis

  • High-Performance Computing (HPC) Server
  • Workstations with Genome Analysis Software

[V] Protein Separation & Characterization

FPLC

  • Purifies proteins based on size, charge, or affinity.
  • Used for enzymes, antibodies, recombinant proteins.

[VI] High-Throughput Proteomics

Mass Spectrometry

  • Analyzes molecules based on mass-to-charge ratio.
  • Used for proteomics, lipidomics, metabolomics.