Phenotypic and Genotypic Characterization of Wetland Bacteria with Potential Biotechnological Applications | Shiv Nadar University

Phenotypic and Genotypic Characterization of Wetland Bacteria with Potential Biotechnological Applications

Bacteria have played a crucial role in biotechnology because of their small size, structural simplicity and rapid division rate with broad metabolic competence. Evaluation of uncharacterized microbes from biodiversity-rich sites can be utilized for biotechnological advancement. To get more insight into their unique attributes, population dynamics, functional role, and possible applications, a detailed characterization of the microbes is required. In this study, we isolated more than 100 bacterial species from the wetland soil and water samples from which we identified 50 isolates by 16S rRNA gene sequencing analysis. After identification of the isolates, a few morphotypes were selected for detailed studies including phenotypic and genotypic characterization. Deinococcus indicus strain DR1 was selected to study bacterial pleomorphism and to identify the factors which affect the morphological alteration. Unique attributes such as arsenic resistance, variation in bacterial shape, and UV-radiation resistance, D. indicus was selected for whole genome sequencing. During genome mining, it was found that D. indicus contains arsenic operon genes and a crucial enzyme required for bacterial resistance for arsenic is structurally different from the known classes of arsenic reductases. We studied the enzyme in detail by molecular modelling and structure prediction methods. The second set of bacteria studied were from the genus Exiguobacterium. We further examined the potential of these bacteria to degrade polystyrene. Janthinobacterium lividum, a beta-proteobacterium, which produces a dark violet-coloured pigment named violacein, was studied in detail. Violacein, a small molecule has shown tremendous potential as an antimicrobial agent. We extensively studied the mode of action of violacein against Gram-positive pathogens as it has shown remarkable therapeutic potential and can be used further for several industrial applications including pharmaceutical and agriculture sectors.

Department: 
Life Sciences
Year: 
2018
Student Name: 
Deepika Chauhan
Faculty Advisor: 
Co-Faculty Advisor: 

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